Ettan™ LC System provides liquid chromatography for biomolecular characterization and micropurification and Fraction Collector Frac-950 is an automated fraction collector for reliable and flexible collection of fractions based on time or volume from ÄKTA™design chromatography systems.
Ettan™ LC System provides liquid chromatography for biomolecular characterization and micropurification and Fraction Collector Frac-950 is an automated fraction collector for reliable and flexible collection of fractions based on time or volume from ÄKTA™design chromatography systems.
Ettan™ LC is designed for separations with microbore, narrow-bore, fast LC, and analytical columns and combines with electrospray mass spectrometers for high-performance LC-MS.
- accurate, reproducible flow rates and gradients.
- reliable, on-line gradient monitoring.
- high-sensitivity, multiple wavelength detection.
Ettan™ LC systems are developed from the ÄKTA™ design chromatography platform and contain inert, biocompatible flow paths with minimized dead volumes to ensure high sample recovery.
Ettan™ LC
The system includes Pump P-905, optimized for low flow rates. Monitor pH/C-900 provides on-line gradient monitoring, as well as useful information for troubleshooting. Monitor UV-900 provides optimal UV-Vis detection in the range 190-700 nm. Columns are connected directly into the flow cell of Monitor UV-900, maximizing sensitivity and resolution by eliminating dead volumes. UNICORN™ software for control and evaluation is supplied with the system. Options for sample fractionation and autosampling include Frac-950.
TECHNICAL SPECIFICATIONS
Ettan LC System
Flow rate range 1 µl/min-2 ml/min
Typical flow rate,
gradient mode > 40 µl/min
Pressure range 0-35 MPa (350 bar, 5075 psi)
Wavelength range 190-700 nm, 3 wavelengths simultaneously
Solvent compatibility All commonly used chromatographic solvents
Size (W × H × D) 450 × 610 × 480 mm
Weight 55 kg
Fraction Collector Frac-950
Frac-950 offers two independent ways of preventing sample loss and the highest flexibility in the choice of collection mode. Fractions are collected in microplates (over 384 fractions, up to 2.5 ml per fraction), a variety of test tube sizes (up to 250 ml per fraction). Collection is in volume or time mode and different fraction sizes can be collected during different stages of a separation. In standard fraction collection mode, fractions can be collected in 96-well microplates (up to four) and 12, 18, and 30 mm standard tubes.
To deal with large fraction volumes, preparative mode allows fraction collection in eighty 30 mm standard tubes, twenty 250 ml bottles, or in 30 funnels, which connect to practically any vessel.
Automatic peak fractionation, based on peak detection using slope or level sensing, minimizes peak dilution and cross-contamination. Event marks correlate the fractions with the chromatogram. A recycle function enables collection from repetitive runs.
The instrument comes with:
- Ettan LC instrument
- Fraction Collector Frac-950
- Software Unicorn
- PC, ready installed
As an extra option the Autosampler A-905 can be provided.
6 months warranty.
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